NOB1 is essential for the survival of RKO colorectal cancer cells

Figure 1 Lentivirus-mediated siRNA decreased NOB1 expression in RKO cells. A: Infection efficiency was estimated 4 d after infection at MOI of 10. Green fluorescent protein (GFP) expression in infected cells was observed under light and fluorescence microscopy, respectively. Light micrograph (left); Fluorescent micrograph (right) (× 200); B: Total RNA was extracted 5 d after infection, and relative NOB1 mRNA expression was determined by qRT-PCR. GAPDH expression was used as a loading control. Data are presented as mean ± SD of three independent experiments. ^aP < 0.05 vs scr-siRNA; C: Total cellular protein was extracted 7 d after infection and determined by western blot analysis using antibodies against NOB1. GAPDH was used as an internal control. MOI: Multiplicity of infection; GFP: Green fluorescent protein; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.