Overexpression of pim-3 and protective role in lipopolysaccharide-stimulated hepatic stellate cells

Figure 4 Effect of ablation of pim-3 on hepatic stellate cells-T6 cell apoptosis. Cells were treated with si-pim3, LPS or si-pim3 plus LPS for 48 h, and apoptosis was detected by flow cytometry followed by annexin V-FITC/PI double staining. A: Representative flow cytometry following Annexin V-FITC and PI staining; B: Data are expressed in a histogram in order to reflect the apoptosis change among the six groups. Data are expressed as mean ± SD. n = 3, ^aP < 0.05 LPS vs control; ^bP < 0.01 versus control; ^cP < 0.01 vs LPS group.