Overexpression of pim-3 and protective role in lipopolysaccharide-stimulated hepatic stellate cells

Figure 3 Effect of si-pim3 on pim-3 expression. Cells were treated at different times and pim-3 protein was detected at 48 h by western blotting. Lane 1: control; Lane 2: liposomes; Lane 3: si-pim3; Lane 4: scrambled; Lane 5: si-pim3 plus LPS; Lane 6: LPS. Upper panel, representative Western blotting results; lower panel, relative expression levels of proteins, normalized against GADPH. Data are expressed as mean ± SD (n = 3 ). ^bP < 0.01 vs control, ^dP < 0.01 LPS vs other groups.