Overexpression of pim-3 and protective role in lipopolysaccharide-stimulated hepatic stellate cells

Figure 2 Overexpression of pim-3 in lipopolysaccharide-stimulated hepatic stellate cells-T6 cells. Cells were treated at different times, with 1 μg/mL lipopolysaccharide for 0, 3, 6, 12, 24 and 48 h, and samples were collected at the same final time of 48 h. mRNA and protein level of pim-3 expression was detected by RT-PCR and Western blotting, respectively. A: Representative mRNA expression; B: Representative protein expression; C: Relative level of pim-3 was normalized to value obtained for β-actin mRNA expression; D: Relative level of pim-3 was normalized to value obtained for GADPH protein expression. The data are expressed in line chart in order to reflect the expression change over time. Data are expressed as mean ± SD (n = 3). ^aP < 0.05 vs 0 h; ^bP < 0.01 vs 0 h.