Expression of renal Oat5 and NaDC1 transporters in rats with acute biliary obstruction

Figure 3 NaDC1 protein expression in homogenates. Renal homogenates (20 μg proteins) (A) and brush border membranes (10 μg proteins) (C) from kidneys of Sham and BDL rats were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (8.5%) and blotted onto nitrocellulose membranes. NaDC1 was identified using a non-commercial polyclonal antibody as described in Materials and Methods. Densitometric quantification of NaDC1 (B and D). Sham levels were set at 100%. Each column represents mean ± SE from experiments carried out in triplicate on four different homogenates and brush border membranes preparations for each experimental group, ^aP < 0.05 vs Sham.