Brewers&rsquo; rice modulates oxidative stress in azoxymethane-mediated colon carcinogenesis in rats

doi:10.3748/wjg.v21.i29.8826

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 7, 2015; 21(29): 8826-8835
Published online Aug 7, 2015. doi: 10.3748/wjg.v21.i29.8826

Table 2 Nucleotide sequence of polymerase chain reaction primers (obtained from GenBank database)

Primer name[Accession number]	Oligonucleotides (5’-3’)Sequence
GSK3β	F: GGGCACCAGAGCTGATCTTT
[NM_032080]	R: GCCGAAAGACCTTCGTCCA
Beta-catenin	F: CGTGGAAGCTGGTGGGATG
[AF121265]	R: TTCCTGCTTAGTCGCTGCAT
NF-κB	F: AGAGGATGTGGGGTTTCAGG
[NM_001276711.1]	R: GCTGAGCATGAAGGTGGATG
iNOS	F: GTACCCTCAGTTCTGTGCCT
[NM_012611.3]	R: TGTTGCGTTGGAAGTGTAGC
Nrf2	F: TCTGACTCCGGCATTTCACT
[NM_031789.2]	R: CCCCAGAAGAATGTGTTGGC
HO-1	F: CTAGAGCAGGACATGGCCTT
[NM_012580.2]	R: GCCTTCTGCGCAATCTTCTT
ACTB¹	F: CCACCCGCGAGTACAACC
[NM_031144.3]	R: TCAGGATGCCTCTCTTGCTC
B2M¹	F: CCCACCCTCATGGCTACTTC
[NM_012512.2]	R: GATGAAAACCGCACACAGGC
RPLP1¹	F: CAAGGTGCTCGGTCCTTCC
[NM_001007604.2]	R: GAGCCTTTGCAAACAAGCCA

¹Housekeeping gene. ACTB: β-actin; B2M: Beta-2 microglobulin; GSK3β: Glycogen synthase kinase 3β; HO-1: Heme oxygenase-1; iNOS: Nitric oxide synthase, inducible; NF-κB: Nuclear factor-kappa B; Nrf2: Nuclear factor E2-related factor 2; RPLP1: Ribosomal protein, large, P1.

Citation: Tan BL, Norhaizan ME, Huynh K, Yeap SK, Hazilawati H, Roselina K. Brewers’ rice modulates oxidative stress in azoxymethane-mediated colon carcinogenesis in rats. World J Gastroenterol 2015; 21(29): 8826-8835
URL: https://www.wjgnet.com/1007-9327/full/v21/i29/8826.htm
DOI: https://dx.doi.org/10.3748/wjg.v21.i29.8826