TLR4 upregulates CBS expression through NF-κB activation in a rat model of irritable bowel syndrome with chronic visceral hypersensitivity

Figure 9 Inhibition of nuclear factor-κB reverses toll-like receptor 4-induced upregulation of cystathionine β synthetase expression in cultured dorsal root ganglion cells. A: Western blotting analysis for p65 expression in DRG neurons incubated with LPS for 8 h, 12 h and 24 h (n = 3 for each group, ^aP < 0.05, ^bP < 0.01, vs CON; B: LPS notably upregulated CBS expression at 12 h and 24 h (n = 3 for each group, ^cP < 0.05, compared with CON); C: PDTC significantly downregulated CBS expression (n = 4 for each group, ^fP < 0.01, vs NS, two-sample t-test); D: AOAA did not alter the upregulation of p65 induced by LPS incubation for 12 h. AOAA: O-(carboxymethyl)-hydroxylamine hemihydrochloride.