TLR4 upregulates CBS expression through NF-κB activation in a rat model of irritable bowel syndrome with chronic visceral hypersensitivity

Figure 7 Inhibition of nuclear factor-κB suppresses cystathionine β synthetase expression. A: Immunofluorescence analysis of p65 expression in CBS-positive, colon-specific DRG neurons. Bar = 50 μm; B: Western blotting analysis for CBS expression after PDTC treatment in NCI rats (n = 6 for each group, ^aP < 0.05, vs NS); C: In contrast, PDTC treatment did not significantly alter TLR4 expression in NCI rats (n = 6 for NS, n = 7 for PDTC, two-sample t-test). PDTC: Pyrrolidine dithiocarbamate.