TLR4 upregulates CBS expression through NF-κB activation in a rat model of irritable bowel syndrome with chronic visceral hypersensitivity

Figure 6 Inhibition of toll-like receptor 4 reduces nuclear p65 expression in neonatal colonic inflammation rats. A: Immunofluorescence analysis of TLR4 expression in p65-positive, colon-specific DRG neurons; Total protein (n = 4) (B) and nuclear protein (n = 5) (C) were used for p65 western blotting in NCI rats (both ^aP < 0.05, vs CON). Western blotting analysis for p65 from (D) total (n = 5) and (E) nuclear (n = 4) protein after CLI095 treatment in NCI rats (both ^aP < 0.05, vs CON). Bar = 50 μm.