Ameliorative effects of lutein on non-alcoholic fatty liver disease in rats

Figure 7 Lutein supplementation improved insulin signaling in rat liver. Effects of lutein on the mRNA expression of IRS2 (A), PI3K (B), and GLUT2 (C) in rat liver (n = 4). Total RNA was extracted from rat livers using TRIzol. IRS2, PI3K, and GLUT2 expression was analyzed by Real-Time RT-PCR. β-actin mRNA was quantified as an endogenous control. IRS2, PI3K, and GLUT2 are presented as fold changes relative to the control. Effect of lutein on the protein expression of hepatic IRS2 (D), PI3K (E), and GLUT2 (F) in rats (n = 3). After the rats were treated with lutein for 45 d, hepatic lysates were prepared and immunoblotted with corresponding antibodies. Blotting with anti β-actin was used as a protein loading control. Data are expressed as the mean ± SD. ^aP < 0.05 vs normal diet (ND) group; ^cP < 0.05 vs the high-fat diet (HFD) group.