Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitro

Figure 7 Levels of the inflammatory mediators high mobility group box 1 protein, IL-1β, IL-6 and TNF-α in the supernatant of the THP-1 cells co-cultured with SW480 cells. After pretreatment with EP (5 mmol/L, 1 h), the SW480 cells were treated with 1 μg/mL LPS and co-cultured with THP-1 cells for 24 h. The levels of the inflammatory mediators HMGB1, IL-1β, IL-6 and TNF-α in the supernatant of the THP-1 cells co-cultured with SW480 cells were detected by ELISA (A-D). Compared with the LPS-untreated group, LPS increased the levels of HMGB1, IL-1β, IL-6 and TNF-α in the THP-1 cells, but EP downregulated these levels. Similarly, HMGB1 A-box also decreased the secretion of HMGB1, IL-1β, IL-6 and TNF-α in the THP-1 cells compared with the mock-transfection group. The data are expressed as the mean ± SD and are derived from three independent experiments, which were each performed in duplicate. The means with letters above them are significantly different (^aP < 0.05, ^bP < 0.01 vs control).