Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitro

Figure 5 Levels of the inflammatory mediators HMGB1 protein, IL-1β, IL-6 and TNF-α in the SW480 cell supernatant. After pretreatment with EP (5 mmol/L, 1 h), the SW480 cells were treated with LPS (1 μg/mL, 24 h). The levels of the inflammatory mediators HMGB1, IL-1β, IL-6 and TNF-α in the supernatant of the SW480 cells were detected by ELISA (A-D). Compared with the LPS-untreated group, LPS increased the levels of HMGB1, IL-1β, IL-6 and TNF-α in the LPS-stimulated SW480 cells, but EP downregulated the levels of HMGB1, IL-1β, IL-6 and TNF-α in the SW480 cells activated by LPS. By contrast, HMGB1 A-box failed to decrease the secretion of HMGB1, IL-1β, IL-6 and TNF-α compared with the mock-transfection group. The data are expressed as the mean ± SD and are derived from three independent experiments, which were each performed in duplicate. The means with letters above them are significantly different (^aP < 0.05, ^bP < 0.01 vs control).