Effect of endogenous cholecystokinin on the course of acute pancreatitis in rats

Table 1 Effect of pancreatic rest or stimulation on the recovery of the pancreas after acute pancreatitis

Parameters	Control	AP-C	AP-R	AP-S	AP-R/S
Pancreatic wet weight (mg/rat)	1120 ± 30	698 ± 581	881 ± 3712	1074 ± 6923	1079 ± 6623
Pancreatic contents
Protein (mg/pancreas)	132 ± 8	50 ± 91	89 ± 41	131 ± 1823	126 ± 1323
DNA (mg/pancreas)	4.2 ± 0.2	1.7 ± 0.31	3.3 ± 0.112	3.5 ± 0.42	4.1 ± 0.42
Protein/DNA (mg/mg)	31.1 ± 0.8	31.3 ± 2.2	27.2 ± 1.312	37.7 ± 1.43	31.3 ± 1.33
Amylase
(103 SU/pancreas)	91.2 ± 17.7	12.3 ± 5.21	46.7 ± 7.812	67.7 ± 13.423	73.4 ± 12.723
(SU/mg protein)	681 ± 105	240 ± 491	520 ± 962	502 ± 722	509 ± 782
(103 SU/mg DNA)	20.4 ± 3	7.0 ± 1.21	14.2 ± 3.22	20.0 ± 3.72	17.6 ± 2.52
Lipase
(103 U/pancreas)	11.6 ± 1.2	4.5 ± 1.21	8.0 ± 0.612	11.4 ± 1.92	12.4 ± 1.62
(U/mg protein)	123.3 ± 22.7	77.4 ± 8.01	86.3 ± 5.91	91.7 ± 6.32	97.9 ± 7.12
(103 U/mg DNA)	3.7 ± 0.6	2.6 ± 0.41	2.7 ± 0.11	3.4 ± 0.32	3.1 ± 0.32
Insulin content
(nmol/pancreas)	20.2 ± 1.2	12.8 ± 0.81	18.9 ± 1.52	16.8 ± 0.62	17.2 ± 0.52
(nmol/mg DNA)	4.95 ± 0.22	8.56 ± 1.101	5.56 ± 0.572	5.25 ± 1.002	4.80 ± 0.552

¹Significant difference vs control;

²Significant difference vs AP-C;

³Significant difference vs AP-R. Values are the mean ± SE of 6-8 rats. At 24 h after induction of acute hemorrhagic pancreatitis by retrograde intraductal infusion of 40 μL/100 g body weight of 3% NaTc, rats were divided into four groups. Rats in the AP-C group received 2 mL/kg body weight saline twice daily for 10 d; the AP-R group received 50 mg/kg body weight CCK receptor antagonist loxiglumide twice daily for 10 d; the AP-S group received 25 mg/kg body weight protease inhibitor camostat twice daily for 10 d; and the AP-R/S group received 50 mg/kg body weight loxiglumide twice daily for the first 5 d followed by 25 mg/kg body weight camostat twice daily for the next 5 d. Rats were fed ad libitum. On day 12 at 24 h after the last treatment and an overnight fasting, pancreatic exocrine function and histological examination of the pancreas were performed.