TLR2 and TLR4 polymorphisms influence mRNA and protein expression in colorectal cancer

Table 2 Polymerase chain reaction-restriction fragment length polymorphism conditions, primers sequences, restriction enzyme and fragment sizes

Polymorphisms	Primers (5’-3’)	Enzymes	Fragment (bp)	Ref.
		T°/time
TLR2-196 to -174del	F: CACGGAGGCAGCGAGAAA	-	ins: 286	[59]
	R: CTGGGCCGTGCAAAGAAG		del: 264
TLR4 +896A/G	F: AGCATACTTAGACTACCACCTCGATG	BstXI	A: 131	[60]
(rs4986790)	R: GTTGCCATCCGAAATTATAAGAAAAG	37 °C/3 h	G: 108; 23
TLR4 -1607T/C	F: TTTGTATAATTTGACTACCATTGCGT	HhaI 37 °C/3 h	T: 139	[30]
(rs10759932)	R: CATTTTTTCACATCTTCACCAGC		C: 117; 22

ins: Wild-type allele (insertion); del: Polymorphic allele (deletion); bp: Base pairs; T^o: Temperature; F: Forward; R: Reverse.