Abundance and significance of neuroligin-1 and glutamate in Hirschsprung’s disease

Figure 2 Both the abundance and density of neuroligin-1 on paraffin-embedded sections (A, B, C) and glutamate on longitudinal muscles with adherent myenteric plexus (D, E, F) are highest in ganglionic segment (A, D), moderate in transitional zone (B, E) and lowest in aganglionic segment (C, F). The black arrows show the positively stained ganglion cells which are expressed between longitudinal muscle and circular muscle both on paraffin-embedded sections (A, B, C) and on longitudinal muscle with adherent myenteric plexus (D, E, F). Immunohistochemical staining, magnification × 400. Bars: 50 μm. Glu: Glutamate.