Abundance and significance of neuroligin-1 and glutamate in Hirschsprung’s disease

Figure 1 Both in ganglionic segment and aganglionic segment, neuroligin-1 (A, D, red) is expressed in the same position (merged, C, F, yellow) where glutamate is expressed (B, E, green). The expressed abundance and density were lower in aganglionic segment (D, E, F) than in ganglionic segment (A, B, C). White arrows show ganglion cell with positive stain with a fusiform or triangular shape. Double immunofluorescence staining, magnification × 400. Bars: 50 μm. Glu: Glutamate.