Abundance and significance of neuroligin-1 and glutamate in Hirschsprung&rsquo;s disease

doi:10.3748/wjg.v21.i23.7172

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 21, 2015; 21(23): 7172-7180
Published online Jun 21, 2015. doi: 10.3748/wjg.v21.i23.7172

Table 2 Detailed information of antibodies and primers

Antigen	Primary antibody	Dilution	Applications
Neuroligin-1	Goat-anti-human polyclonal	1/100	Detect Nlgn-1 with immunofluorescence on LMMP
Neuroligin-1	Goat-anti-human polyclonal	1/50	Detect Nlgn-1 with Immunohistochemistry on paraffin-embedded sections
Neuroligin-1	Goat-anti-human polyclonal	1/200	Detect Nlgn-1 with Western-blot
Glutamate	Mouse-anti-human monoclonal	1/200	Detect Glu with immunofluorescence on LMMP
Glutamate	Mouse-anti-human monoclonal	1/200	Detect Glu with Immunohistochemistry on LMMP
Glutamate	Mouse-anti-human monoclonal	1/400	Detect Nlgn-1 with Western-blot
β-actin	Rat-anti-human polyclonal	1/2000	Western-blot internal reference
Secondary antibody	Dilution	Applications	Source
Donkey anti-goat Texas Red Secondary	1/500	Label Nlgn-1 with immunofluorescence	ZSGB-BIO China
Goat anti-mouse FITC Secondary	1/200	Label Glu with immunofluorescence	ZSGB-BIO China
Horseradish Peroxidase-conjugated goat-anti-rat IgG	1/500	Detect β-actin with Western-blot	SANTA CRUZ United States
Horseradish Peroxidase-conjugated rabbit-anti-goat IgG	1/1000	Detect Nlgn-1 with Western-blot	SANTA CRUZ United States
Horseradish Peroxidase-conjugated goat-anti-mouse IgG	1/1000	Detect Glu with Western-blot	SANTA CRUZ United States
Primer	Primer sequence (5’→3’)	Annealing temperature (°C)	Product size (bp)
Neuroligin-1	F: GCAAGACCAGAGCAGAGACT	59	314
	R: CACCACCAAAGAATCCAATGTT
β-actin	F: AGCGAGCATCCCCCAAAGTT	60	285
	R: GGGCACGAAGGCTCATCATT

F: Forward primer; R: Reverse primer.

Citation: Wang J, Du H, Mou YR, Niu JY, Zhang WT, Yang HC, Li AW. Abundance and significance of neuroligin-1 and glutamate in Hirschsprung’s disease. World J Gastroenterol 2015; 21(23): 7172-7180
URL: https://www.wjgnet.com/1007-9327/full/v21/i23/7172.htm
DOI: https://dx.doi.org/10.3748/wjg.v21.i23.7172