Proportions of acetyl-histone-positive hepatocytes indicate the functional status and prognosis of cirrhotic patients

Figure 2 Representative images of hematoxylin and eosin staining (magnification × 20) and immunohistochemistry (magnification × 200) staining of H2AK5ac, H3K9/K14ac and H3K27ac in different Child-Turcotte-Pugh grade tissue microarray slides. Nuclear brown staining is considered positive expression. From Child-Turcotte-Pugh (CTP) grades A to C, the proportions of hepatocytes that express each marker gradually increase (A). The proportions of H2AK5ac⁺, K3K9/K14ac⁺ and H3K27ac⁺ hepatocytes are statistically significant in different CTP grades for the 611 patients (B) (P < 0.001) and 152 follow-up patients (C) (P < 0.001). The cutoff of 70% acetyl-histone-positive hepatocytes divided the patients into two groups with lower or higher CTP grades. HE: Hematoxylin and eosin.