Neuron-glial antigen 2 overexpression in hepatocellular carcinoma predicts poor prognosis

Figure 2 Neuron-glial antigen 2 protein and mRNA expression in human hepatic carcinoma and matched adjacent non-tumorous tissues. A: The relative neuron-glial antigen 2 (NG2) protein expression levels were measured by Western blot. NG2 expression significantly increased in hepatic carcinoma tissue (T) compared with matched adjacent non-tumorous tissue (ANT) (n = 30). The horizontal lines represent the mean NG2 protein expression level, and the P value is from the paired samples t-test; B: Representative Western blot images of NG2 protein expression in 8 sets of human hepatic carcinoma (T) and matched ANT tissues. β-actin expression was used as a loading control; C: The relative NG2 mRNA expression levels significantly increased in hepatic carcinoma tissue (T) compared with matched ANT (n = 30). The mRNA expression levels were measured by RT-PCR. The horizontal lines represent the mean NG2 mRNA expression levels, and the P value is from the paired samples t-test; D: NG2 mRNA expression levels were compared in 8 pairs of hepatic carcinoma (T) and matched ANT tissues. ^aP < 0.05.