Low RASSF6 expression in pancreatic ductal adenocarcinoma is associated with poor survival

Figure 2 Quantitative real-time polymerase chain reaction evaluation of RASSF6 expression in pancreatic ductal adenocarcinoma tissues. A: RASSF6 mRNA levels in pancreatic ductal adenocarcinoma (PDAC) tissues were significantly decreased compared with its levels in chronic pancreatitis tissues (P < 0.001); B: Groups were made according to the immunohistochemical results, and the consistency between immunohistochemical results and PCR results was evaluated through t test. Result showed that immunohistochemical scores were in keeping with RASSF6 mRNA levels (^bP < 0.001); C, D: RASSF6 mRNA and protein expression levels were evaluated by quantitative real-time polymerase chain reaction and Western blot in 16 cases with strong RASSF6 immunohistochemical staining. Even in these PDAC tissues with strongly positive RASSF6 staining, both the mRNA expression (C: P = 0.033) and protein levels (D: P = 0.049) were still less than para-tumor tissues.