New NCI-N87-derived human gastric epithelial line after human telomerase catalytic subunit over-expression

Figure 4 Evaluation of the expression of epithelial gastric markers by the parental NCI-N87 and NCI-hTERT-clones 5 and 6 cell lines. A: Slot-blot of 100 μg of total protein extracts for detection of mucin 5AC (MUC5AC) with the anti-MUC5AC mAb (1:1000 diluted); B: Western blot of 100 μg of total protein extracts separated in a 12.5% (v/v) SDS-PAGE, with the anti-Pg5 mAb (1:800 diluted) and the anti-HGL pAb (1:800 diluted).