Alternative splicing of VEGFA, APP and NUMB genes in colorectal cancer

doi:10.3748/wjg.v21.i21.6550

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 7, 2015; 21(21): 6550-6560
Published online Jun 7, 2015. doi: 10.3748/wjg.v21.i21.6550

Table 3 Primers for polymerase chain reaction-restriction fragment length polymorphism

Gene	Primers	Sequence (5'-3')	Products (bp)
GAPDH	Forward	FAM-TGAAGGTCGGAGTCAACGGA	225
GAPDH	Reverse	CCTGGAAGATGGTGATGGGAT	225
VEGFA	Forward	FAM-TGAGCTTCCTACAGCACAAC	206/338/410
VEGFA	Reverse	TCGATGGTGATGGTGTGGTG	206/338/410
APP	Forward	FAM-CCTACGAAGAAGCCACAGAG	162/330/387
APP	Reverse	GGGCATGTTCATTCTCATCC	162/330/387
NUMB	Forward	FAM-TGCTCCGATGACCAAACCAG	157/301
NUMB	Reverse	CACCTCTTCTAACCATCGGTC	157/301

Citation: Zhao YJ, Han HZ, Liang Y, Shi CZ, Zhu QC, Yang J. Alternative splicing of VEGFA, APP and NUMB genes in colorectal cancer. World J Gastroenterol 2015; 21(21): 6550-6560
URL: https://www.wjgnet.com/1007-9327/full/v21/i21/6550.htm
DOI: https://dx.doi.org/10.3748/wjg.v21.i21.6550