CD97 promotes gastric cancer cell proliferation and invasion through exosome-mediated MAPK signaling pathway

Figure 1 Identification of transfectants with stable CD97 knockdown. A: RT-PCR analysis was used to assess CD97 mRNA levels of SGC/wt, SGC/ns and SGC/kd. This result showed a decreased CD97 level in SGC/kd compared to SGC/wt and SGC/ns; B: The -80 kDa CD97 protein was detected by western blot analysis in total cellular extracts of SGC/wt, SGC/ns and SGC/kd; C: Western blot analysis showed a significant decrease in CD97 protein in SGC/kd. Data are expressed as mean ± SD. ^aP < 0.05 vs SGC/wt or SGC/ns. SGC/wt: Wild-type gastric cancer cells SGC-7901; SGC/ns: Non-silencing vector bearing cells; SGC/kd: Stable CD97 knockdown cells generated from SGC-7901.