SGK1 inhibits cellular apoptosis and promotes proliferation via the MEK/ERK/p53 pathway in colitis

Figure 6 Efficiency of serum-and-glucocorticoid-inducible-kinase-1 silencing after serum-and-glucocorticoid-inducible-kinase-1 siRNA transfection and co-immunoprecipitation of serum-and-glucocorticoid-inducible-kinase-1 and mitogen-activated protein kinase kinase 1. A, B: The efficiency of SGK1 silencing the subsequent dysregulation of p-ERK and ERK in HCT-116 cells. The efficiency of SGK1 silencing was assessed by the ratio of SGK1/GAPDH. The level of ERK phosphorylation was assessed by the ratio of p-ERK/ERK; C, D: The efficiency of SGK1 silencing and the following dysregulation of p-ERK and ERK in IEC-6 cells. The NC group served as controls. GAPDH served as the internal control; E, F: SGK1 was co-immunoprecipitated with MEK1 on IECs. HCT-116 and IEC-6 cell lysates were subjected to co-immunoprecipitation with anti-SGK1 or control IgG and were detected with anti-MEK1 antibodies. MEK1 was co-immunoprecipitated with SGK1. ^aP < 0.05 and ^bP < 0.01 vs negative controls.