SGK1 inhibits cellular apoptosis and promotes proliferation via the MEK/ERK/p53 pathway in colitis

Figure 5 Intestinal epithelial cell apoptosis and proliferation analyzed by TUNEL and CCK-8 assays. A: After the indicated treatment for 48 h, the cells were TdT-UTP nick end labeled and photographed with a fluorescent microscope. Scale bar = 200 μm. The TUNEL-positive cells are colored green; B: Quantitation of TUNEL-positive intestinal epithelial cells (IECs) was performed on all groups. All values are mean ± SD from 6 independent images in each group; C: For the CCK-8 assay, IECs were transfected with SGK1 siRNA or negative control siRNA (NC) as described above. OD450 values indicate the absorbance of IECs post-transfected at a wavelength of 450 nm. The OD450 values of IEC-6 and HCT-116 cells are shown in C and D, respectively. ^aP < 0.05 and ^bP < 0.01 vs negative controls.