Thrombospondin peptide ABT-898 inhibits inflammation and angiogenesis in a colitis model

Figure 2 Effects of the TSP-1 mimetic peptide ABT-898 on microvascular density in colons with dextran sodium sulfate-induced colitis. Combined immunohistochemistry against the endothelial markers CD31 and MECA 34 (brown staining) was performed to analyze the vascular density in colitic lesions (A-D). Evaluation of vascular density in TSP-1^-/- colonic sections from mice treated with 5% glucose (A), and ABT-898 (B), WT mice controls (C), and the ABT-898 peptide (D). Sections from mice receiving the ABT-898 peptide showed a significant decrease in microvascular density (MVD) vs controls (E) (P < 0.0001 for TSP-1^-/- colons and P = 0.0048 for WT). ^aP < 0.0001 vs control. TSP-1^-/-: TSP-1^-/- controls; TSP-1^-/-p: TSP-1^-/- treated with ABT898; WT: WT control; WTp: WT treated with ABT-898 peptide.