Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression

Figure 4 Butein treatment decreased mucosal expression of phosphorylated signal transducer and activator of transcription 3 and matrix metalloproteinase-9 expression in interleukin-10^-/- mice. A: Immunohistochemical staining for phosphorylated signal transducer and activator of transcription 3 (pSTAT3) and matrix metalloproteinase (MMP)-9 detected nuclei of epithelial cells or stromal cells of interleukin (IL)-10^-/- mice (black arrows); B: Western blot analysis revealed reduced pSTAT3 protein expression in intestinal epithelial cells of butein-treated IL-10^-/- mice (n = 3). PBS: Phosphate-buffered saline.