MiR-451 inhibits proliferation of esophageal carcinoma cell line EC9706 by targeting CDKN2D and MAP3K1

Figure 3 MiR-451 inhibits the proliferation of EC9706 cells by targeting CDKN2D and MAP3K1. A: CDKN2D and MAP3K1 protein levels were detected by Western blot assay. Western blot assay showed that transfection of CDKN2D-siRNAs, MAP3K1-siRNAs and miR-451 mimics inhibited the expression of CDKN2D and MAP3K1, respectively; B: CDKN2D silencing and miR-451 overexpression inhibited the cell proliferation. CCK8 array was used to assess EC9706 proliferation; C: MAP3K1 silencing and miR-451 overexpression inhibited the cell proliferation. CCK8 array was used to assess EC9706 proliferation; D: Co-transfection of si-CDKN2D and si-MAP3K1 inhibited the proliferation of EC9706 cells. CCK8 array was used to assess EC9706 proliferation; E: CDKN2D, MAP3K1 silencing and miR-451 overexpression reduced the growth of colonies of EC9706 cells. Colony formation assay was used; F: CDKN2D, MAP3K1 silencing and miR-451 overexpression induced EC9706 cell apoptosis. The cell apoptosis was assessed using flow cytometry assay; G: CDKN2D, MAP3K1 silencing and miR-451 overexpression suppressed EC9706 cell invasiveness. The cell invasion was assessed using transwell assay; H: CDKN2D silencing and the overexpression of miR-451 significantly increased the percentage of cells in the G1 phase and decreased the percentage of cells in the S phase (^aP < 0.05 vs control group). The cell cycle was assessed using flow cytometry assay.