MiR-451 inhibits proliferation of esophageal carcinoma cell line EC9706 by targeting CDKN2D and MAP3K1

Figure 1 CDKN2D and MAP3K1 are direct targets of miR-451 in EC9706 and KYSE150 cells. A: The putative miR-451 binding sequence for the CDKN2D 3'-untranslated region (3'-UTR). The 3'-UTR of CDKN2D contains a seed region for miR-451; B: The putative miR-451 binding sequence for the MAP3K1 3'-UTR. The 3'-UTR of MAP3K1 contains a seed region for miR-451; C: Western blot analysis of CDKN2D expression in transfected cells. Transfection of miR-451 mimics resulted in a significant reduction of CDKN2D protein expression in EC9706 and KYSE150 cells. β-actin was used as a reference; D: Western blot analysis of MAP3K1 expression in transfected cells. Transfection of miR-451 mimics resulted in a significant reduction of MAP3K1 protein expression in EC9706 and KYSE150 cells. β-actin was used as a reference; E: MiR-451 significantly decreased the luciferase activity of CDKN2D 3’-UTR-Wt in EC9706 and KYSE150 cells; F: MiR-451 significantly decreased the luciferase activity of MAP3K1 3’-UTR-Wt in EC9706 and KYSE150 cells (^aP < 0.05 vs control group).