Downregulation of rho-associated protein kinase 1 by miR-124 in colorectal cancer

Figure 3 Expression of miR-124 and rho-associated protein kinase 1 in colorectal cancer cell lines. A: Representative Western blot of rho-associated protein kinase 1 (ROCK1) expression in the human colonic mucosa epithelial cell line (NCM460) and colorectal cancer (CRC) cell lines (HCT116, HT29 and SW620); B: Densitometry analysis of ROCK1 expression relative to GAPDH; Quantitative real time-PCR data for C: miR-124; and D: ROCK1 mRNA. All experiments were repeated three times.