Autophagy in anti-apoptotic effect of augmenter of liver regeneration in HepG2 cells

Figure 3 3-MA suppresses autophagic activity in HepG2 cells. 3-MA (final concentration, 750 ng/mL) was added to Dulbecco’s modified Eagle’s medium (DMEM) when the medium was changed to serum-free medium. Autophagy was observed at 24 h after serum deprivation. A: HepG2 cells were transfected with the GFP-LC3 plasmid and cultured in DMEM for 6 h, followed by treatment with serum-free medium. Green puncta were observed using an inverted fluorescence microscope (Nikon Eclipse E800); B: Total cell lysates were subjected to Western blot analysis using anti-LC3 and anti-β-actin antibodies. 3-MA: 3-Methyladenine; LC3: Light chain three.