Hepatitis B virus preS1 deletion is related to viral replication increase and disease progression

Table 7 Comparison of fluorescence resonance energy transfer-based real-time polymerase chain reaction vs direct sequencing protocol for detection of deletion variants n (%)

Direct sequencing	Result for FRET-based real-time PCR
	Only wild type (n = 13)1	Mixed infection (n = 10)2
Only wild type	13 (100)	3
Mixed infection	0	7 (70)

¹Thirteen samples that were identified as only wild type infection by FRET-based real-time PCR were subjected to a direct sequencing protocol;

²The samples identified as mixed infection with both wild type and deletion variant by FRET-based real-time PCR were subjected to a direct sequencing protocol. FRET-based real-time PCR: Fluorescence resonance energy transfer-based real-time polymerase chain reaction.