Down-regulation of aquaporin3 expression by lipopolysaccharide via p38/c-Jun N-terminal kinase signalling pathway in HT-29 human colon epithelial cells

Figure 5 Effect of p38/c-Jun N-terminal kinase inhibitors on lipopolysaccharide-induced down-regulation of aquaporin 3 expression in HT-29 cells. Cells were incubated with 20 μg/mL lipopolysaccharide for 12 h, after pre-treatment with inhibitors for 30 min. Aquaporin 3 protein expression was determined by Western blot; β-actin served as a loading control. Data represent one of three independent experiments, and quantitative data are presented as mean ± SD from three independent experiments, ^aP < 0.05 vs control.