Down-regulation of aquaporin3 expression by lipopolysaccharide via p38/c-Jun N-terminal kinase signalling pathway in HT-29 human colon epithelial cells

Figure 4 p38/c-Jun N-terminal kinase inhibitors specifically blocked p38/c-Jun N-terminal kinase phosphorylation in HT-29 cells induced by lipopolysaccharide. Cells were pre-treated with the p38 kinase inhibitor SB203580 (10 μg/mL) or the c-Jun N-terminal kinase (JNK) inhibitor SP600125 (20 μg/mL) for 30 min, before incubation with 20 μg/mL lipopolysaccharide for 60 min. Immunoblot analysis was then performed for phospho-p38 kinase (p-p38), p38 kinase, phospho-JNK (p-JNK), and JNK. ^bP < 0.01 vs cells without inhibitor treatment.