Down-regulation of aquaporin3 expression by lipopolysaccharide via p38/c-Jun N-terminal kinase signalling pathway in HT-29 human colon epithelial cells

Figure 2 Down-regulation of aquaporin 3 mRNA and protein expression by lipopolysaccharide in HT-29 cells. A: Dose-dependent decrease in aquaporin 3 (AQP3) mRNA and protein expression by lipopolysaccharide (LPS). Cells were incubated in media supplemented with LPS (0, 10, 20, 50 and 100 μg/mL) for 12 h; B: Time-dependent decrease in AQP3 mRNA and protein expression by LPS. Cells were incubated in media supplemented with 100 μg/mL LPS for various durations (0, 3, 6, 12 and 24 h). The mRNA and protein levels of AQP3 were determined by reverse-transcription PCR and Western blot, respectively. For Western blot, β-actin served as a loading control. Data are presented as mean ± SD of three independent experiments; ^aP < 0.05, ^bP < 0.01 vs control; NS: Not significant.