MicroRNA-185-5p mediates regulation of SREBP2 expression by hepatitis C virus core protein

Figure 4 Upregulation of sterol regulatory element binding protein 2 by Hepatitis C virus core protein involves miR-185-5p. A: 30 nmol/L miR-185-5p mimic or negative control miRNA was co-transfected with pmirGLO-SREBP2 3’-UTR luciferase constructs into HepG2 cells overexpressing core protein for 48 h, and the relative luciferase activity was detected (n = 3); B: In the presence of pmirGLO-SREBP2 3’-UTR, 30 nmol/L of miR-185-5p mimic was co-transfected with either HCV core protein or the indicated null vector into HepG2 cells for 48 h, and the relative luciferase activity was then detected (n = 3). SREBP2: Sterol regulatory element binding protein 2; UTR: Untranslated region.