MicroRNA-185-5p mediates regulation of SREBP2 expression by hepatitis C virus core protein

Figure 2 Hepatitis C virus core protein decreases the expression of miR-185-5p. A: The 3’ untranslated region of sterol regulatory element binding protein 2 was co-transfected with control vector (middle) or core protein (right) into HepG2 cells, after 48 h, the relative luciferase activity was detected; Renilla luciferase activity acted as internal control for each transfected well (n = 3, ^bP < 0.01 core vs control); B: 48 h after transfection of a vector expressing the core protein, expression of selected miRNAs was analyzed by quantitative real-time PCR according to the prediction results. RNU6B was used to normalize the mature miRNAs expression levels (n = 3, ^aP < 0.05 core vs control).