Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice

Figure 6 Hydrogen-rich water inhibited connexin 32 expression in the liver tissue. A: Representative photographs of connexin 32 (Cx32) expression in the liver. The acetaminophen (APAP)-challenged mice had more Cx32 immunopositive staining, as indicated by the brown color, while treatment with hydrogen-rich water (HRW) can significantly reduce Cx32 immunostaining; B: The Cx32 IHC staining score indicated that hydrogen therapy could significantly lower the staining score and reduce its activation and expression; C: The relative Cx32 mRNA levels in the three groups; D: Western blot analysis for the protein content of JNK and cytochrome P4502E (CYP2E1) proteins in response to APAP and HRW treatment in the liver. β-actin was used as an internal control (n = 6, mean ± SD, ^aP < 0.05 vs NS control group; ^bP < 0.05 vs APAP + NS group, and ^cP < 0.05 vs HRW control group). NS: Normal saline.