Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice

Figure 5 Hydrogen-rich water facilitates hepatocyte proliferation after acetaminophen challenge. A: Representative immunohistochemistry staining of BrdU, Ki67 and PCNA in the saline plus acetaminophen (APAP) as well as hydrogen-rich water (HRW) plus APAP treatment mice. The quantification of hepatocyte proliferation was assessed by calculating the positive cells observed by microscopy (magnification × 200); B: Western blot analysis for the protein content of cyclin D1 and PCNA proteins in response to APAP and HRW treatment in the liver. β-actin was used as an internal control (n = 6, mean ± SD, ^aP < 0.05 vs NS control group, ^bP < 0.05 vs APAP+NS group, and ^cP < 0.05 vs HRW control group). NS: Normal saline.