Early activated hepatic stellate cell-derived molecules reverse acute hepatic injury

Figure 5 Hepatic stellate cell-CM (5d) treatment improves hepatocellular necrosis and immune cell infiltration in APAP-injured liver tissue. All animals were fasted overnight before APAP treatment. Acute liver injury (ALI) mice were sacrificed 24 h after systemic vehicle or hepatic stellate cell (HSC)-CM treatment. Liver samples were subjected to histological analysis after HE staining. Microscopic low-power fields of liver tissue are shown after vehicle (A), HSC-CM (5d) (B) and HSC-CM (P3) (C) treatment. Necrotic area is indicated by arrowheads. 1: centrilobular vein, 2: portal vein. Bar = 100 μm. Scores were determined by semi-quantitative histological examination (D). Data are the mean ± SE of the mean of 10 random high-power fields per animal. ^aP < 0.05, ^bP < 0.01 vs control. Bar = 100 μm. NS: Not significant.