Early activated hepatic stellate cell-derived molecules reverse acute hepatic injury

Figure 3 Infusion of hepatic stellate cell lysate provides a trend toward increased survival in acetaminophen-induced acute liver injury. All animals were fasted overnight before acetaminophen (APAP) treatment. Mice administered APAP were treated by intravenous injection of hepatic stellate cells (HSCs) or HSC lysates from the same cell mass (0.2 mL, about 2 × 10⁶ cells). Control mice received vehicle (0.2 mL, high-glucose DMEM). Kaplan-Meier survival analysis of APAP-administered mice treated with cell transplants or lysates is performed. Time points of interventions are stated above survival plots. The results are cumulative data of two independent experiments (n = 13 per group) using different batches of HSCs. The number of death mice during survival analysis in each group: 9 in vehicle, 10 in HSC (5d) transplant, 6 in HSC (5d) lysate, 9 in HSC (P3) transplant, and 8 in HSC (P3) lysate. P-values were determined by the Kaplan-Meier analysis. ^aP < 0.05 vs control. NS: Not significant.