Increase in apoptosis by combination of metformin with silibinin in human colorectal cancer cells

Figure 3 Combined metformin and silibinin treatment enhances AMP-activated protein kinase phosphorylation. COLO 205 cells were treated with silibinin and metformin in combination or alone. The protein samples were then probed using an antibody against phosphorylated AMP-activated protein kinase (p-AMPK) (A) or phosphorylated mammol/Lalian target of rapamycin (p-mTOR) (B). The data are expressed as mean ± SE (n = 6 for each group). ^bP < 0.001 vs Control. Con: Control; Veh: Vehicle; Sili: silibinin; Met: Metformin; M + S: Metformin and silibinin.