Increase in apoptosis by combination of metformin with silibinin in human colorectal cancer cells

Figure 2 Combined metformin and silibinin treatment inhibits protein kinase B phosphorylation by enhancing phosphatase and tensin homolog expression. Proteins isolated from COLO 205 cells were probed using an antibody against phosphatase and tensin homolog (PTEN) (A) or phosphorylated protein kinase B (p-Akt) (B). The data are expressed as mean ± SE (n = 6 for each group). ^aP < 0.01 and ^bP < 0.001 vs Control. Con: Control; Veh: Vehicle; Sili: Silibinin; Met: Metformin; M + S: Metformin and silibinin.