Thalidomide ameliorates portal hypertension via nitric oxide synthase independent reduced systolic blood pressure

Figure 4 Portal vein ligation does not increase hepatic or aortic tumor necrosis factor alpha mRNA expression. Pre-hepatic portal hypertension was induced in wild type mice by partial ligation of the portal vein. 0-24 h post ligation livers (A) and thoracic aortas (B) were harvested and quantified for tumor necrosis factor (TNF) mRNA by reverse transcription-polymerase chain reaction. TNFα was not changed within livers and aorta following portal vein ligation (PVL). Figures are representative of three experiments. Hepatic (C) and aortic (D) TNFα expression was unchanged 0-4 d post PVL. Line graphs represent mean ± SE. n = 5 mice.