Methylation of IRAK3 is a novel prognostic marker in hepatocellular carcinoma

Figure 1 Gene expression and methylation analyses of IRAK3 and GLOXD1. A: Gene expression levels of IRAK3, GLOXD1, and GAPDH (an internal reference gene) were analyzed by RT-PCR in normal controls, THLE-3 cells, 6 HCC cell lines, and HCC cell lines treated with 5DAC; B: Methylation status of IRAK3, GLOXD1, and COL2A (an internal reference gene) was analyzed by MS-PCR with methylated primers in normal controls, THLE-3 cells, 6 HCC cell lines, and HCC cell lines treated with 5DAC. Positive and negative are peripheral blood lymphocyte (PBL) DNA in vitro treated with or without CpG methyltransferase (M.SssI). 1/5 positive and 1/10 positive indicate 1:5 and 1:10 dilution of the positive control.