Copyright
©The Author(s) 2015.
World J Gastroenterol. Apr 7, 2015; 21(13): 3876-3887
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3876
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3876
Figure 3 Fucosylation is enhanced in cancer stem cell-like cells fractions of pancreatic cancer cells.
A: Phase-contrast images of monolayer (left panel) and sphere cells (right panel) derived from Panc1 cells; B: Results of flow cytometric analysis in monolayer and sphere Panc1 cells using CD24 and CD44 antibodies. CD24+/CD44+ cells were increased in sphere cells compared with monolayer cells; C: Aleuria aurantia lectin (AAL) or Aspergillus oryzae lectin (AOL) lectin blot of monolayer and sphere Panc1 cells; D: AAL or AOL lectin blot of CD24high/CD44high or CD24low/CD44low Panc1 cells obtained by cell sorting. Each region is indicated as a square field in (B); E: Comparison of sphere cell-forming ability between Panc1-P and Panc1-RG; F: AAL or AOL lectin blot of monolayer and PSN-1 cells. Results are expressed as mean ± SD; bP < 0.01 vs Panc1-P cells. CBB: Coomassie brilliant blue.
- Citation: Terao N, Takamatsu S, Minehira T, Sobajima T, Nakayama K, Kamada Y, Miyoshi E. Fucosylation is a common glycosylation type in pancreatic cancer stem cell-like phenotypes. World J Gastroenterol 2015; 21(13): 3876-3887
- URL: https://www.wjgnet.com/1007-9327/full/v21/i13/3876.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i13.3876