Fucosylation is a common glycosylation type in pancreatic cancer stem cell-like phenotypes

Figure 2 Fucosylation is enhanced in gemcitabine-treated pancreatic cancer cells. A: Differential glycan profiles of Panc1-P and Panc1-RG cells were analyzed by lectin microarray. The fluorescence intensity of each lectin was normalized to the intensity of wheat germ agglutinin; B: Aleuria aurantia lectin (AAL) or Aspergillus oryzae lectin (AOL) lectin blot of Panc1-P and Panc1-RG. Coomassie brilliant blue (CBB) staining confirms equal protein loading among lanes; C: Cell-surface fucosylated glycoproteins in Panc1-P and Panc1-RG cells were assessed by flow cytometry using Ulex europaeus agglutinin (UEA)-I, AAL, and Pholiota squarrosa lectin (PhoSL) lectins; D: Fucosylation-related gene expression, determined by real-time reverse transcription PCR in Panc1-P and Panc1-RG cells, and expressed relative to that of Panc1-P cells; E: Flow cytometric analysis represented gemcitabine treatment increased the ratio of CD24⁺/CD44⁺ cells in PK59 cells; F: AAL or AOL lectin blot of PK59 cells with (+) or without (-) gemcitabine treatment. All results are expressed as mean ± SD; ^aP < 0.05 vs Panc1-P cells.