Methylation of IRAK3 is a novel prognostic marker in hepatocellular carcinoma

doi:10.3748/wjg.v21.i13.3960

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 7, 2015; 21(13): 3960-3969
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3960

Table 1 Primer and probe sequences for reverse-transcription polymerase chain reaction, methylation-specific polymerase chain reaction, pyrosequencing, and quantitative methylation-specific polymerase chain reaction

Primer	Sequence (5'→3')	Amplicon (bp)
RT-PCR
IRAK3-Forward	ATGCAGTGTAAGAAGCATTGGA	247
IRAK3-Reverse	GCAGGTAGTGAATGGCTTTGG
GLOXD1-Forward	CCCTTCCTACCCGGCTTCA	122
GLOXD1-Reverse	TGGAACCAGCGCAAAAGTGT
Pyrosequencing
GLOXD1-Forward	GAAGGGAGGTTTAGTGTTTAAGGA	242
GLOXD1-Reverse	AGCTGGACATCACCTCCCACAACGCCACCCCAACCAAAAACA
Universal primer	AGCTGGACATCACCTCCCACAACG-Biotin
Sequencing primer	AGGTTTAGTGTTTAAGGAT
MSP/Q-MSP
IRAK3-Forward	AGGAGATCGTTTAGTCGTGGGGTAC	110
IRAK3-Reverse	ACCTCTACGATAAAAACGAAACTACCG
IRAK3-Probe	CTACCGAAACAAACAAAATA
GLOXD1-Forward	AGGATGTGATTAGGCGTGAGGTTC	122
GLOXD1-Reverse	AAAAAAACGAAACCCGTAACTCCG
GLOXD1-Probe	FAM-CGCTACTCTTTCCCC

Allele-specific primer sequences for MSP and Q-MSP are the same. RT-PCR: Reverse transcription-polymerase chain reaction; MSP: Methylation-specific polymerase chain reaction; Q-MSP: Quantitative MSP.

Citation: Kuo CC, Shih YL, Su HY, Yan MD, Hsieh CB, Liu CY, Huang WT, Yu MH, Lin YW. Methylation of IRAK3 is a novel prognostic marker in hepatocellular carcinoma. World J Gastroenterol 2015; 21(13): 3960-3969
URL: https://www.wjgnet.com/1007-9327/full/v21/i13/3960.htm
DOI: https://dx.doi.org/10.3748/wjg.v21.i13.3960