High persistence rate of hepatitis B virus in a hydrodynamic injection-based transfection model in C3H/HeN mice

Figure 2 Quantification of intrahepatic hepatitis B virus DNA in the hydrodynamically injected mice. C3H/HeN or C57BL/6 mice were hydrodynamically injected with 10 μg of pAAV/HBV1.2 plasmid, and the livers of hydrodynamically injected C3H/HeN (n = 3) or hydrodynamically injected C57BL/6 (n = 3) mice were collected at 8 wk or 46 wk after HI. Naive C3H/HeN (n = 3) or C57BL/6 (n = 3) mice were controls (ctrl). A: HBV DNA/mGAPDH in C3H/HeN or C57BL/6 mouse liver total DNA with or without 0.5 μL DpnI digestion at 8 wk post HI; B: HBV DNA/mGAPDH in C3H/HeN or C57BL/6 mouse liver total DNA with or without 0.5 μL DpnI digestion at 46 wk post HI. HBV: Hepatitis B virus; HI: Hydrodynamic injection.