Degradation of intestinal mRNA: A matter of treatment

Figure 3 Time course of RNA integrity from isolated RNA from muscle and submucosal/mucosal tissue with an initial separation or with separation after storage. A: In the experiment “before” the gut from rat was first rinsed with PBS and separated into muscle and submucosal/mucosal layer and then the samples were stored in medium on ice or at room temperature (RT). In the experiment “after” the tissue of the gut was stored in medium on ice or at RT and at different points in time samples were washed with PBS and separated in muscle and submucosal/mucosal layer. The RNA integrity number was measured. Data are presented as the mean ± SE; ^aP < 0.05 and n = 6; B: A fitting of the factors: temperature, tissue, time, species and tissue separation of the gut in muscle and submucosal/mucosal layer before or after incubation was calculated. To identify the impact factors upon the RIN, an analysis of variance (ANOVA) at ^aP < 0.05 significant level was used and n = 6. RIN: RNA integrity number.